細菌および肝ミクロゾームのアゾならびにニトロ還元酵素の比較

概要

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As a part of studies on the participation of intestinal flora in the metabolism of food additives, some properties of azo- and nitro-reductase systems of intestinal bacteria and liver microsomes were compared. The bacterial reductase was prepared from the lyophilized cells of E. coli by ultrasonic disruption. Most of azo- and nitro-reductase activity which required flavin mononucleotide and nicotinamide adenine dinucleotide phosphate was found in the 105000×g supernatant. The bacterial reductase was much more effective than liver microsomes in the reduction of water-soluble sulfonated azo dyes and p-nitrobenzoic acid. However, fat-soluble azo dyes, such as Oil Yellow AB and OB, were not reduced by either of the enzyme systems. Some difference in the substrate specificity for azo dyes, was observed between the two enzyme systems and there was a remarkable difference in the inhibitory effect by CO, KCN, O_2,and chelating agents.
社団法人日本薬学会の論文
1975-11-25