Nucleotides. XX. Synthesis and Properties of Poly-2-alkyladenylic Acids. III. Interactions with Poly (X)

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Poly-2-methyladenylic acid (poly (m^2A)), poly-2-ethyladenylic acid (poly (e^2A)) and poly-2-isopropyladenylic acid (poly (iso-pr^2A)), which are polyadenylic acid (poly (A)) analogues bearing alkyl groups at the C (2)-position of the adenine moiety, form 1 : 1 complexes with polyxanthylic acid (poly (X)) under the same conditions where poly (A) forms both 1 : 1 and 1 : 2 complexes. In contrast to the base pairing between poly (A) and poly (X), these poly-2-alkyladenylic acids cannot, for steric resons, form normal Watson-Crick type complexes involving the amino group and the N (1)-atom, but coordinate instead with the N (7)-position. Relative rates of complex formation between poly-2-alkyladenylic acids and poly (X) vary with the nature of the alkyl substituents. The half-times for the complex formation were determined for the poly (m^2A)-poly (X), poly (e^2A)-poly (X) and poly (iso-pr^2A)-poly (X) systems as 10min, 〜7h and 〜9h, respectively. In the presence of 0.1M Na^+, pH7.0,the poly (m^2A)・poly (X) complex does not dissociate below 95℃, but the poly (e^2A)・poly (X) and poly (iso-pr^2A)・poly (X) complexes show cooperative dissociation at T_ms of 84.2 and 69.7℃, respectively. The ultraviolet and circular dichromic spectra of the complexes are also presented and compared with those of poly (A)・poly (X).
社団法人日本薬学会の論文
1983-11-25