Structural Studies of the Interactions of Normal and Abnormal Human Plasmins with Bovine Basic Pancreatic Trypsin Inhibitor
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概要
- 論文の詳細を見る
Catalytic activity of human plasmin is inhibited by bovine basic pancreatic trypsin inhibitor (BPTI, also known as aprotinin). In spite of increased interest in the function of BPTI as an inhibitor of plasmin, the 3-D structure of the plasmin-BPTI complex has not yet been determined. Therefore, in the present paper, the structure of the plasmin-BPTI complex was constructed by the homology modeling method, which provided information about the high affinity of plasmin for BPTI. Moreover, normal mode analyses of free plasmin, free BPTI and the plasmin-BPTI complex were carried out to investigate the changes in dynamics following complex formation.After study of the plasmin-BPTI interaction, we also investigated the binding of BPTI with abnormal plasmin, theoretically and experimentally. The result showing that BPTI binds to abnormal plasmin in the same way as it does to normal plasmin supports the previous finding that the difference between normal and abnormal plasmins is very small and that the abnormality is localized to the catalytic site.
- 社団法人日本薬学会の論文
- 1999-03-15
著者
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Umeyama Hideaki
School of Pharmacy, Kitasato University
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Takeda-Shitaka Mayuko
School of Pharmacy, Kitasato University
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Takeda‐shitaka M
School Of Pharmaceutical Sciences Kitasato University
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Takeda-shitaka Mayuko
Riken Systems And Structural Biology Center
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Takeda Shitaka
School Of Pharmaceutical Sciences Kitasato University
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Takeda-shitaka Mayuko
School Of Pharmaceutical Sciences Kitasato University
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Takeda-shitaka Mayuko
School Of Pharmacy Kitasato University
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KAMIYA Kenshu
School of Science, Kitasato University
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MIYATA Toshiyuki
National Cardiovascular Center Research Institute
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OHKURA Naoki
National Cardiovascular Center Research Institute
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MADOIWA Seiji
Division of Thrombosis and Hemostasis, Institute of Hematology, Jichi Medical School
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SAKATA Yoichi
Division of Thrombosis and Hemostasis, Institute of Hematology, Jichi Medical School
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Kamiya Kohei
Dep. Of Pharmacognosy And Natural Product Chemistry Fac. Of Pharmaceutical Sciences Kobe Gakuin Univ
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Kamiya Kohei
Department Of Pharmacognosy And Natural Product Chemistry Faculty Of Pharmaceutical Sciences Kobe Ga
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Ohkura N
National Cardiovascular Center Res. Inst. Osaka
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Sakata Y
Division Of Thrombosis And Hemostasis Institute Of Hematology Jichi Medical School
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Sakata Yoichi
Division Of Cell And Molecular Medicine Center For Molecular Medicine Department Of Medicine
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Madoiwa Seiji
Department Of Medicine Cell And Molecular Medicine Center For Molecular Medicine Jichi Medical Unive
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Madoiwa S
Division Of Thrombosis And Hemostasis Institute Of Hematology Jichi Medical School
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Madoiwa Seiji
Division Of Hemostasis And Thrombosis Institute Of Hematology Jichi Medical Scool
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Kamiya Kohei
Dep. Of Pharmacognosy And Natural Product Chemistry School Of Pharmaceutical Sciences Kobe Gakuin Un
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Kamiya Kenshu
School Of Science Kitasato University
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Umeyama Hideaki
Dep. Of Biomolecular Design School Of Pharmaceutical Sciences Kitasato Univ.
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Umeyama Hideaki
School Of Pharmaceutical Sciences Kitasato University
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Miyata T
National Cardiovascular Center Research Institute
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SAKATA Yoichi
Division Cell and Molecular Medicine, Jichi Medical School, Tochigi
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