Dynamic Character of Human Growth Hormone and Its Receptor: Normal Mode Analysis

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概要

• 論文の詳細を見る

• Human growth hormone (hGH) induces dimerization of its binding protein (hGHbp). hGH binds to the first hGHbp (bpl) on site 1, and then the hGH-bpl heterodimer complex binds to the second hGHbp (bp2) on site 2. Although the interactions of hGH and hGHbps have been studied from different viewpoints, few studies from a dynamic viewpoint have been reported. Especially, since in the SCOP domain database hGHbp is classified as two clear immunoglobulin-like domains, it is of interest to understand how hGH interacts with the hGHbp domains. Therefore, we carried out normal mode analysis (NMA) of free hGH, free bpl, free bp2, and the hGH-bpl heterodimer complex, as well as the hGH-bpl-bp2 ternary complex to investigate how the dynamics of the proteins change before and after forming the complexes. NMA showed that the domain motion between the N-terminal and the C-terminal domains of free bpl markedly decreased after binding to hGH, and that the domain motion of bp2 decreased similarly after binding to the hGH-bpl heterodimer complex. The present study demonstrates that hGH regulates the inter-domain motions of both hGHbps.

• 社団法人日本薬学会の論文
• 2003-07-01

著者

• Umeyama Hideaki

  School of Pharmacy, Kitasato University

• Takeda-Shitaka Mayuko

  School of Pharmacy, Kitasato University

• Takeda‐shitaka M

  School Of Pharmaceutical Sciences Kitasato University

• Takeda-shitaka Mayuko

  School Of Pharmaceutical Sciences Kitasato University

• Takeda-shitaka Mayuko

  School Of Pharmacy Kitasato University

• NOJIMA Hiroyuki

  School of Pharmaceutical Sciences, Kitasato University

• KAMIYA Kenshu

  School of Science, Kitasato University

• Kurihara Youji

  School of Pharmaceutical Sciences, Kitasato University

• WATANABE Tomoko

  School of Pharmaceutical Sciences, Kitasato University

• SUMIKAWA Hiromi

  Central Research Laboratories, Ajinomoto Co., Inc.

• Kamiya Kohei

  Dep. Of Pharmacognosy And Natural Product Chemistry Fac. Of Pharmaceutical Sciences Kobe Gakuin Univ

• Kamiya Kohei

  Department Of Pharmacognosy And Natural Product Chemistry Faculty Of Pharmaceutical Sciences Kobe Ga

• Kurihara Y

  School Of Pharmaceutical Sciences Kitasato University

• Kamiya Kohei

  Dep. Of Pharmacognosy And Natural Product Chemistry School Of Pharmaceutical Sciences Kobe Gakuin Un

• Sumikawa H

  Central Research Laboratories Ajinomoto Co. Inc.

• Sumikawa Hiromi

  Central Research Laboratories Ajinomoto Co. Inc.

• Kamiya Kenshu

  School Of Science Kitasato University

• Umeyama Hideaki

  Dep. Of Biomolecular Design School Of Pharmaceutical Sciences Kitasato Univ.

• Umeyama Hideaki

  School Of Pharmaceutical Sciences Kitasato University

• Kurihara Youji

  School Of Pharmaceutical Sciences Kitasato University

• Watanabe Tomoko

  School Of Pharmaceutical Sciences Kitasato University

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