Highly Increased Plasma Concentrations of the Nicked Form of .BETA.2 Glycoprotein I in Patients with Leukemia and with Lupus Anticoagulant. Measurement with a Monoclonal Antibody Specific for a Nicked Form of Domain V.
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概要
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β2-Glycoprotein I (β2G-PI) consists of five tandem repeated domains (I, II, III, IV, and V). The nicked form of (N-β2CPI ) which was cleaved by plasmin in vitro at Lys 317-Thr 318 in domain V, showed reduced affinity for the negatively charged phospholipids, especially cardiolipin (CL). Recently, the N-β2 was detected in the plasma of patients with disseminated intravascular coagulation syndrome (DIC) by an immunological method. In the present study, we prepared monoclonal antibodies for the nicked form, and demonstrated that the concentrations of this form of β2GPI, which were analyzed by a sandwich ELISA using two specially prepared monoclonal antibodies, were significantly increased in the plasma of patients with leukemia (n=51, mean±SD: 162.0±118.3ng/ml) and with lupus anticoagulant (LA) (n=40, mean±SD: 3, 041.5±16, 579.7ng/ml), compared to the normals (n=33, mean±SD: 1.04±1.54ng/ml). We found a significant correlation between the concentrations of N-β2GPI and those of typical molecular markers for a fibrinolytic state such as plasmin-α2 plasmin inhibitor complex (PIC) and D-dimer in patients with leukemia, but not in patients with LA. These results suggested that the generation of N-β2GPI was caused by plasmin in the patients with leukemia, and by unknown proteases in the patients with LA. In the patients with LA, the levels of N-β2GPI tended to be higher in those without thrombosis than in those with thrombosis.
- 社団法人 日本生化学会の論文
著者
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OHKURA Naoki
National Cardiovascular Center Research Institute
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KATO Hisao
National Cardiovascular Center Research Institute
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Shiku Hiroshi
Second Department Of Internal Medicine Mie University School Of Medicine
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Kohno Isao
Narita R&d Center Iatron Laboratories Inc.
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Wada Hideo
Second Department Of Internal Medicine Mie University School Of Medicine
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Inuzuka Kimiko
Narita R&D Centel, lation Laboratories Inc.
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Itoh Yumiko
Narita R&D Centel, lation Laboratories Inc.
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- Highly Increased Plasma Concentrations of the Nicked Form of .BETA.2 Glycoprotein I in Patients with Leukemia and with Lupus Anticoagulant. Measurement with a Monoclonal Antibody Specific for a Nicked Form of Domain V.