Site-Specific Induction of Metallothionein in N-Nitrosodimethylamine-Treated Rat Liver

スポンサーリンク

概要

• 論文の詳細を見る

• The effects of dietary protein and sulfur-containing amino acids on oxidative responses against N-nitrosodimethylamine (NDMA) treatment were investigated in rat liver, focusing on the mechanism of metallothionein (MT) induction. Rats were fed a 10% soybean protein isolate (SPI) diet or SPI supplemented with 0.3% L-methionine (SPI-Met) for 3 weeks after weaning. Rats fed on SPI showed lower glutathione (GSH) levels and higher MT levels in the liver than those fed on SPI-Met. After treatment with NDMA (20 mg/kg, i.p.), MT levels were elevated significantly in both groups, whereas GSH levels were only elevated in the SPI group. Pathologically, necrosis and hemorrhage were seen in the livers of NDMA-treated rats of both dietary groups, suggesting oxidative damage caused by NDMA treatment. Immunological histochemistry using anti-MT antibody showed that most of the MT was distributed in the cytosol region of all the rat groups. The ratios of the MT-positive cells were correlated to MT levels that were chemically analyzed. It was notable that the localization of MT staining in the hepatic lobules was altered after NDMA injection. The MT staining was principally observed around the central vein before injection, whereas it was more markedly observed at the peripheral region after NDMA treatment. These results indicate that the synthesis of hepatic MT basically occurs around the central vein with oxygen stress caused by insufficient oxygen supplement and/or stimulated oxygen consumption. NDMA could be oxidatively metabolized at the peripheral region with sufficient supplemental oxygen from the artery, resulting in oxidative stress and stimulation of MT synthesis there. Thus, distribution of tissue MT might vary depending on the site of oxidative stress.

• 公益社団法人日本薬学会の論文
• 2003-04-01

著者

• Yasutake Akira

  Biochemistry Section Department Of Basic Medical Sciences National Institute For Minamata Disease

• Yasutake Akira

  Biochemistry Section National Institute For Minamata Disease

• Mizutani Yusuke

  Department Of Veterinary Pathology Tottori University

• Shimada Akinori

  Department Of Comparative Pathology Faculty Of Veterinary Medicine Hokkaido University

• Taniguchi Misako

  Division Of Food And Nutrition Nakamura Gakuen University

• Shimada Akinori

  Department of Veterinary Pathology,Tottori University

関連論文

• The influence of Parachlorella beyerinckii CK-5 on the absorption and excretion of methylmercury (MeHg) in mice
• Demethylation of Methylmercury in Human Neuroblastoma, Glioblastoma and Liver Cells
• Resistance of human brain microvascular endothelial cells in culture to methylmercury : cell-density-dependent defense mechanisms
• Involvement of metallothionein (MT) as a biological protective factor against carcinogenesis induced by benzo[a]pyrene (B[a]P)
• Resistance of metallothionein-III null mice to cadmium-induced acute hepatotoxicity
• Protective role of metallothionein in benzo[a]pyrene-induced DNA damage
• Experimental Scuticociliatosis in Japanese Flounder (Paralichthys olivaceus) Infected with Miamiensis avidus: Pathological Study on the Possible Neural Routes of Invasion and Dissemination of the Scuticociliate inside the Fish Body
• Pathological Study on the Scuticociliatosis Affecting Farmed Japanese Flounder (Paralichthys olivaceus) in Japan
• Distribution of the Inflammatory Lesions in the Central Nervous System of Dogs Affected with Disseminated and Ocular Form of Granulomatous Meningoencephalomyelitis
• Suppression of fibroblast growth factor-2 expression : Possible mechanism underlying methylmercury-induced inhibition of the repair of wounded monolayers of cultured human brain microvascular endothelial cells
• Methylmercury Retards the Repair of Wounded Monolayer of Human Brain Microvascular Endothelial Cells by Inhibiting Their Proliferation without Nonspecific Cell Damage
• Neurotoxic action of inorganic mercury injected in the intraventricular space of mouse cerebrum
• Selective and Simple Quantification of Metallothionein III in Mouse Brain
• Kinetic Study on Methylmercury Uptake by Rat Erythrocytes (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
• Effects of Iron Overload on Hepatic and Renal Metallothionein Levels in Rats
• Mechanism of Methylmercury Efflux from Rat Astrocytes (Proceedings of the 19th Symposium on Toxicology and Environmental Health)
• Simple Method for Methylmercury Estimation in Biological Samples Using Atomic Absorption Spectroscopy
• Reappraisal of the Historic 1959 Cat Experiment in Minamata by the Chisso Factory
• Chronic Effects of Methylmercury in Rats : II. Pathological Aspects
• Chronic Effects of Methylmercury in Rats : I. Biochemical Aspects
• "P-15 Biological Effects of Chronic Exposure to Methylmercury of Low Level.
• Influence of Dietary Levels of Protein and Sulfur Amino Acids on Metabolism of Glutathione and Related Amino Acids in Mice
• Role of Mitochondria in Biotransformation of Methylmercury (Proceedings of the 23rd Symposium on Toxicology and Environmental Health)
• Influence of Dietary Protein Levels on the Acute Toxicity of Methylmercury in Mice (Proceedings of the 21st Symposium on Toxicology and Environmental Health)
• Influence of Dietary Protein Levels on the Subacute Toxicity of Methylmercury in Mice (Proceedings of the 20th Symposium on Toxicology and Environmental Health)
• Influence of Supplementary Sulfur Amino Acids on the Fate of Methylmercury in Mice Fed on a Low Protein Diet
• Studies on Plasma Methylmercury Compound and Its Brain Uptake (Proceedings of the 16 th Symposium on Environmental Pollutants and Toxicology)
• Influence of Low Protein Diet on the Fate of Methylmercury and Tissue Glutathione Levels (Regular Presentations) (Proceedings of the 15 th Symposium on Environmental Pollutants and Toxicology)
• Sex Difference of Acute Toxicity of Methylmercury in Mice (Proceedings of the 14th Symposium on Environmental Pollutants and Toxicology)
• Primary Malignant Peripheral Nerve Sheath Tumor with Eosinophilic Cytoplasmic Globules Arising from the Greater Omentum in a Dog
• Absence of Tumor Promoting Activity of Xylazine in a Two-stage Nasal Carcinogenesis Model in N-bis(2-hydroxypropyl)nitrosamine-treated Rats
• Current Hair Mercury Levels in Japanese for Estimation of Methylmercury Exposure
• Current Hair Mercury Levels in Japanese : Survey in Five Districts
• Acute Phase Effects of Methylmercury on Glutathione Metabolisms (Proceedings of the 20th Symposium on Toxicology and Environmental Health)
• Wistar-Imamichi Rats Exhibit a Strong Resistance to Cadmium Toxicity
• Increase of Calcium Concentration in the Testes of Mice Treated with Rare Earth Metals
• Prevention of Nephrotoxicity of Cisplatin by Repeated Oral Administration of Ebselen in Rats
• Ultrastructural and biochemical studies on the intestinal absorption of a medium-chain triglyceride (MCT), tricaprylin
• Neurobehavioral effects of combined prenatal exposure to low-level mercury vapor and methylmercury
• The enhanced elimination of tissue methylmercury in Parachlorella beijerinckii-fed mice
• Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo
• Chlorella suppresses methylmercury transfer to the fetus in pregnant mice
• TWO CASES OF TELENCEPHALIC AND OPTICUS ASTROGLIOSIS IN LEAD INGESTED CATTLE : PATHO-MORPHOLOGICAL OBSERVATIONS
• Methylmercury inhibits electron transport chain activity and induces cytochrome c release in cerebellum mitochondria
• Induction by mercury compounds of metallothioneins in mouse tissues : inorganic mercury accumulation is not a dominant factor for metallothionein induction in the liver
• Site-Specific Induction of Metallothionein in N-Nitrosodimethylamine-Treated Rat Liver
• Neurobehavioral changes and alteration of gene expression in the brains of metallothionein-I/II null mice exposed to low levels of mercury vapor during postnatal development
• Chronic Low-Dose Methylmercury Administration Decreases Mitochondrial Enzyme Activities and Induces Myopathic Changes in Rats
• Chlorella suppresses methylmercury transfer to the fetus in pregnant mice
• Neurobehavioral changes and alteration of gene expression in the brains of metallothionein-I/II null mice exposed to low levels of mercury vapor during postnatal development
• Effect of dental amalgam on gene expression profiles in rat cerebrum, cerebellum, liver and kidney
• Distribution of mercury in metallothionein-null mice after exposure to mercury vapor : amount of metallothionein isoform does not affect accumulation of mercury in the brain
• Antimicrobial Resistance of Salmonella Serovars Isolated from Beef at Retail Markets in the North Vietnam
• Metallothionein deficiency exacerbates chronic inflammation associated with carcinogenesis in stomach of mice infected with Helicobacter pylori
• Changes in Extracellular Neurotransmitters in the Cerebrum of Familial Idiopathic Epileptic Shetland Sheepdogs Using an Intracerebral Microdialysis Technique and Immunohistochemical Study for Glutamate Metabolism
• Encephalitis in Mice Inoculated Intranasally with an Influenza Virus Strain Originated from a Water Bird.
• Aspiration Pneumonia as a Cause of Neonatal Death in Three Captive Bottlenose Dolphins (Tursiops truncates)
• Connective Tissue-Type Mast Cell Leukemia in a Dog.
• Multiple Osseous Metastases of a Carotid Body Tumor in a Dog

もっと見る 閉じる

スポンサーリンク