Methylmercury inhibits electron transport chain activity and induces cytochrome c release in cerebellum mitochondria
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概要
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The involvement of oxidative stress has been suggested as a mechanism for toxicity caused by methylmercury (MeHg). One of the major critical sites for oxidative stress is the mitochondria. In this research, to clarify the target site in mitochondria affected by MeHg, the individual activities of the mitochondrial electron transport chain (ETC) (I∼IV) were examined in the liver, cerebrum and cerebellum of MeHg-intoxicated rats. In addition, to elucidate the mechanism underlying MeHg toxicity, cytochrome c release, caspase 3 activity and histological study were examined in the cerebrum and cerebellum. The cerebellum was found to be an exclusive tissue in which significant MeHg-induced alterations were observed. The complex II activity in the cerebellum mitochondria significantly decreased after MeHg exposure. Cytochrome c release from mitochondria increased only in the cerebellum by MeHg exposure. However, no significant alterations in caspase 3 activity or histological structure were found in brain tissues. These results suggest that MeHg acts on the constituents of complex II in the cerebellum, and induces mitochondrial dysfunction, leading to a release of cytochrome c from mitochondria. These events were considered to occur at the early stage of MeHg intoxication.
- 2011-06-01
著者
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Yasutake A
National Inst. For Minamata Disease Kumamoto Jpn
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Yasutake Akira
Biochemistry Section National Inst. For Minamata Disease
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Yasutake Akira
Biochemistry Section Department Of Basic Medical Sciences National Institute For Minamata Disease
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Yasutake Akira
National Inst. For Minamata Disease
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Hirayama Kimiko
熊本大学 医学部保健学科
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Mori Nobuko
Kumamoto University College Of Medical Science
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Mori Nobuko
Department Of Applied Bioscience Research Institute For Advanced Science And Technology University O
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Marumoto Masumi
National Institute for Minamata Disease
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Hirayama Kimiko
Department Of Biomedical Laboratory Sciences Faculty Of Life Sciences Kumamoto University
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Marumoto Masumi
National Inst. For Minamata Disease
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