IMMUNOCYTOCHEMICAL LOCALIZATION OF α-ACTININ IN GASTRIC PARIETAL CELLS OF THE RAT
スポンサーリンク
概要
- 論文の詳細を見る
Localization of α-actinin in the rat gastric parietal cell was studied using Lowicryl K4M embedding method as well as cryoultramicrotomy in combination with immunogold technique. These two methods gave similar results, but labeling efficiency was a little higher in cryoultrathin sections than in Lowicryl K4M sections. Labeling for α-actinin occurred in the cytoplasm adjacent to the zonula adherens, around the secretory canaliculus, in the microvillus, and along the basolateral infolding. Little labeling was seen around the tubulovesicle. Quantitative analysis of the distribution of gold particles in Lowicryl K4M sections revealed a significant decrease of α-actinin in the cytoplasm around the canaliculusin the secreting cell in comparison to the resting cell. The present study showed that the gastric parietal cell is different from the intestinal epithelial cell in that it contains α-actinin in the microvilli. The decreaseof α-actinin around the intracellular canaliculus in the secretory state may be related to the morphological alteration of the parietal cell through reorganization of the actin filament meshwork.
- 日本組織細胞化学会の論文
著者
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Ogawa K
Department Of Veterinary Anatomy Graduate School Of Agriculture And Biological Sciences Osaka Prefec
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Fujimoto T
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
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Mizuno M.
Department of Geriatric Medicine, Faculty of Medicine, Kyoto University
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Fujimoto T.
Department of Anatomy, Faculty of Medicine, Kyoto University
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Ogawa K.
Department of Anatomy, Faculty of Medicine, Kyoto University
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Fujimoto T.
Meteorological Research Institute:(present Affiliation)japan Meteorological Agency
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