DISTRIBUTION OF F-ACTIN, FODRIN, AND ANKYRIN IN GASTRIC PARIETAL CELLS OF THE RAT
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概要
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Distribution of F-actin and the membrane skeletal proteins fodrin and ankyrin in gastric parietal cells of the rat was examined by immunofluorescence and immunoelectron microscopic techniques of frozen sections. Rats were treated with gastrin or famotidine, a histamine H_2 receptor antagonist, to stimulate or inhibit secretion, respectively. F-actin stained by rhodamine-phalloidin was seen along the apical and basolateral cell surface and in the intracellular canaliculi (IC). The canalicular staining was more pronounced in gastrin-treated than in famotidine-treated rats. By immunofluorescence microscopy, fodrin was observed along the basolateral cell membrane and around IC in famotidine-treated rats; ankyrin coexisted with fodrin only in the basolateral cell membrane. Following treatment with gastrin IC was expanded and fodrin labeling in IC was less intense. Immunoelectron microscopy showed that fodrin and ankyrin were distributed along the basolateral cell membrane. Fodrin was also seen densely around IC, which distribution became sparser in stimulated cells. Neither fodrin nor ankyrin was found in the tubulovesicles. The present study showed that distribution of both F-actin and fodrin in IC changed according to the cells secretory state and this change might be related to the morphological alterations of the gastric parietal cell during secretion. In addition, coexistence of fodrin and ankyrin was revealed along the basolateral cell membrane.
- 日本組織細胞化学会の論文
著者
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Ogawa K
Department Of Veterinary Anatomy Graduate School Of Agriculture And Biological Sciences Osaka Prefec
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Fujimoto T
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
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Mizuno M.
Department of Geriatric Medicine, Faculty of Medicine, Kyoto University
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Fujimoto T.
Department of Anatomy, Faculty of Medicine, Kyoto University
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Ogawa K.
Department of Anatomy, Faculty of Medicine, Kyoto University
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Fujimoto T.
Meteorological Research Institute:(present Affiliation)japan Meteorological Agency
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