FREEZE-FRACTURE IMAGES OF DISTRIBUTION OF FILIPIN-CHOLESTEROL COMPLEXES IN THE OVIDUCT EPITHELIUM OF MICE

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In order to clarify the distribution of cholesterol in the plasma- and cyto-membranes of the oviduct epithelium of mice, freeze-fracture images of the filipin-treated tissues were observed. The filipin-sterol complexes were distributed in high density on the fractured plasma membranes of both secretory and ciliated cells except for special areas. The limiting membranes of secretory granules of the secretory cell were very rich in complexes. In both types of cells, membranes of nucleus and rough endoplasmic reticulum were virtually free of the complexes, though very small aggregates of a few complexes were infrequently scattered. Most Golgi stacks were labeled slightly with filipin, but small vesicles and a stack in the trans-side showed a fairly large number of the complexes to form small aggregates. No complexes were recognized on the outer and inner mitochondrial membranes of both type cells. Although most of the ciliary membrane contained numerous complexes, only the ciliary necklace portion lacked the complexes. The area of zonula occludens was generally free of the complexes. The membrane enclosed by the strands of zonula occludens was devoid of the complexes, though filipin extensively labeled the membrane surrounding the zonula occludens. Round patches studded beneath the zonula occludens, which might correspond to desmosomes, did not bear the complexes.
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