競合PCR法における増幅過程の数式モデル

概要

論文の詳細を見る
Competitive PCR is a useful method for quantating minute amounts of a target DNA. In this method, a competitor DNA, whose sequence is either homologous or heterologous to the target DNA, is coamplified as an internal standard. The authors experimentally examined features of both types of competitor DNA. When a heterologous competitor was used, the target and the competitor were coamplified with different efficiencies, the one whose initial amount was smaller being more amplified. When a homologous competitor was used, however, both the target and the competitor were coamplified with equivalent efficiency. To analyze this phenomenum theoretically, a simple model of theccoamplification process in which the binding betweentthe DNA templates was the dominant factor in the amplification efficiency was constructed, and the model equations were numerically solved to simulate competitive PCR. The results of the simulation were consistent with those of an actual competitive PCR experiment.
社団法人日本生物工学会の論文
1997-01-25