水銀化合物におよぼす水銀耐性菌の作用に関する研究 : (第2報)水銀耐性細菌による水銀化合物の気化の促進

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The fate of mercury bound to the cells of mercury-resistant Pseudomonas was investigated, Mercury-boud cells were prepared after the organism was incubated in 0.02M phosphate buffer at pH 7.2 containing 100ppm radioactive phenylmercuric acetate (PMA) for 2hr or containing 40ppm mercuric chloride (MC) for 30 min. A mercury compound extracted from PMA-bound cells with 0.1N acetic acid or extracted from MC-bound cells with 0.1N hydrochloric acid was compared with each mercurial before binding by thin layer chromatography. Marked difference was not found between them, which implies that the binding is rather loose one like absorption. When mercury-bound cells were transferred to fresh culture medium and incubated, the disappearance of mercury from culture was observed before the beginning of growth. When mercury-bound cells were fractionated by the Schneider method, mercury was almost found in both cold trichloroacetic acid-soluble fraction and ethanol-water-soluble fraction. The mercury present in these two fractions was markedly decreased compared with that in other fractions during incubation. The disappearance was also observed when the cells were directly incubated with mercurials in culture medium. The disappeared mercury was completely absorbed from gas phase by active carbon which was placed in Conway's microdiffusion apparatus. It was suggested from these results that the vaporization of mercury was stimulated by the organism after the binding of mercurials to the cells. However, nothing is known about the chemical form of vaporized mercury and the mechanism of the vaporization. Evidence was obtained suggesting that the stimulative vaporization was biological. For instance, the vaporization of PMA was stimulated in proportion to the concentration of cells, and inhibited by an addition of PMA more than 150ppm. The vaporization was not observed by heated cells or by cells treated with reagents such as toluene and acetone. The significance of the vaporization was discussed from the viewpoint that the organism plays an important role for reducing the toxicity of mercurials present in its environment.
社団法人日本生物工学会の論文
1968-09-25

水銀化合物におよぼす水銀耐性菌の作用に関する研究 : (第2報)水銀耐性細菌による水銀化合物の気化の促進

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