1,2-プロパンジオールに生育したペニシリウム属糸状菌によるグルクロン酸オリゴマーの生成

概要

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A strain of Penicillium, which was capable of growing on 1,2-propanediol as a sole source of carbon, was isolated from soil. Microscopic observations indicated that it belonged to the division of monoverticillata in Penicillium. When the organism was grown on 1,2-propanediol, uronides were found in the culture filtrate. The maximum amount of the uronides was about 1.1mg per ml as glucuronic acid. The uronides were precipitated from the culture filtrate by the addition of ethanol, and then purified by anion-exchange column chromatography and subsequently by sephadex G-15 column chromatography. Analytical data revealed that the major component of the uronides consisted of glucuronic acid and glucose with the molar ratio of 7 to 1. The molecular weight was estimated to be in the range form 700 to 1500. Polyglucuronide was obtained from cell walls of the organism. The uronic acid content in cell walls was about 5%, and in cells about 2%. The production of oligoglucuronide was discussed on a view it was apparently not attributed to release from cell walls.
社団法人日本生物工学会の論文
1970-09-25