水銀化合物におよぼす水銀耐性菌の作用に関する研究 : (第1報)水銀耐性細菌の分離と, その細胞への水銀化合物の結合

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Mercury-resistant organism was isolated from soil heavily contaminated with phenylmercuric acetate (PMA), and the function of the organism on mercurials including mercuric chloride (MC), PMA and ethylmercuric phosphate (EMP) was investigated. The organism was found to belong to the genus Pseudomonas on the basis of taxonomic observatioin. The growth of the organic was allowed in the presence of MC less than 450 ppm, PMA less then 120 ppm, or EMP less than 20 ppm. These values were approximately a thousand times higher than those obtained by other bacteria. When the organism was incubated in 0.02 M phosphate buffer at pH 7.2 containing ^<203>Hg-labelled MC, the rapid uptake of radioactivity by the organism was observed. ^<203>Hg-labelled PMA was also taken up with a slower rate under the same condition. The maximum amount of mercury taken up by the organism was 2×10^8 atoms per cell in MC, 1×10^8 atoms in PMA, and 0.6×10^8 atoms by EMP. Evidence was obtained suggesting that mercury taken up by the organism was mainly located on the cell surface, when the distribution of radioactive mercury was examined for fractions obtained by differential centrifugation after mechanical disruption of mercury-bound cells.
社団法人日本生物工学会の論文
1968-06-25