Bacillus moriguchiensisのAmylase並びにProtease生産條件に關する研究(第1報) : 酵素生産に及ぼす窒素源の影響(其の1)

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We studied the effect of various nitrogen sources on the amylase and protease production by B. moriguchiensis which was isolated from soil.For the experiments we used the basal medium containing soluble starch 1.0g, MgSO_4・7H_2O 0.1g, nitrogen source 0.1g (as total-N), 1% dry yeast extract (as growth promoting material) 5.0 ml, N/5 phosphate buffer solution (pH=6.0) 25 ml in 100 ml of medium.1) Among the nitrogen sources, NH_4-lactate gave superior result, and then (NH_4)_2HPO_4 was also effective. But NH_4Cl, (NH_4)_2SO_4,NH_4NO_8,KNO_3,NH_4-tartarate, NH_4-acetate, NH_4-butyrate and usea gave no satisfactory result.2) Peptone, dry yeast, defatted soy bean, and wheat-bran were all good nitrogen sources, but casein and rice-bran were not so good as those.3) The bacillus did not grow in media containig the hydrolysates obtained by cooking such proteinous materials as defatted soy bean, wheat-bran, and rice-bran with sulphuric acid because of inhibition by over-hydrolyzation products of carbohydrates contained in them. But peptone and casein which were decomposed with H_2SO_4 geve good result, especially peptone digested with proteolytic enzyme was more effective.4) Among the amino acids used for the test, -viz., asparagine, aspartic acid, glutamic acid, tyrosine, leucine and alnine-alanine and asparagine were good nitrogen sources for the enzyme production by this bacterium.
社団法人日本生物工学会の論文
1954-05-15