麹菌の變異に關する研究 (第5報) B. 自然變異について (1)

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We presupposed nutrient deficiency of culture media to be a cause of natural variation which occured in long continued subcultures of Aspergillns oryzae, and we attempted to select a most suitable medium for preserving this fungus without natural variation. Accordingly, the subcultures of this fungus were continued by using various media to observe the state of variation. The tested media were koji-extract agar, wort agar, koji-extract plus corn-extract agar, wort plus corn-extract agar, rice, wheat-bran, defatted soy-bean and mixture of wheat-bran and defatted soy-bean.As a result of experience, the variation occured somewhat a little frequently in the cultures on grains, natural or properly processed in comparison with those on agar media. Among various agar media, wort plus corn-extract agar caused variation not so frequently as the other.
公益社団法人日本生物工学会の論文
1954-03-15