炭化水素生育酵母のペルオキシゾーム

概要

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Candida yeasts using alkanes as their sole carbon and energy source are industrially important and academically interesting. They can produce many compounds with physiological and metabolic features derived from such unconventional carbon sources and may become an excellent model for investigating fatty acid metabolism in mammalian tissues.We found that a conspicuous number of peroxisomes (microbodies), an organelle, appear in Candida tropicalis cells grown on alkanes. These peroxisomes contain various enzymes, including catalase, four enzymes constituting the fatty acid β-oxidation system, acyl-CoA synthetase, carnitine acetyltransferase, and the key enzymes of the glyoxylate cycle (isocitrate lyase and malate synthase), so they are organelles with important functions in fatty acid metabolism in this yeast.Since development and degradation of peroxisomes as well as the induction and repression of peroxisomal enzymes can be easily controlled by changing the carbon source for growth, these yaste cells could be an excellent tool for studying the localization of specific peroxisomal enzymes in connection with the development of the organelles.The review summarizes practical aspects of Candida yeasts assimilating alkanes and fundamental aspects of peroxisomes from these yeast cells.
公益社団法人日本生物工学会の論文
1985-03-25