キチナーゼ生産細菌の分離と酵素の生産条件および性質

概要

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Chitinase producing becteria were isolated on chitin agar plates from seashore muds of the Ariake Sea in the Kyusyu district of Japan. One strain, no. 12,having potent chitinase activity, was identified as belonging to the genus Vibrio on the basis of taxonomic characteristics. This strain (Vibrio sp. no. 12) produced an inducible chitinase system and the maximum production of chitinase was found at 30℃ for 48hr culture in a medium containing 0.2% colloidal chitin, 0.2% glucose, 0.5% yeast extract and 0.5% peptone. The chitinase system consisted of chitinase and chitobases, which could be separated from each other by column chromatography on DEAE-Sephadex A-25. It seemed that chitinase decomposed colloidal chitin to chitin-oligosacchardies, then to N, N-diacetylchitobiose, which was further hydrolysed to N-acetylglucosamine by chitobiase.
社団法人日本生物工学会の論文
1979-05-25