イネ縞葉枯ウイルスのヒメトビウンカ体内増殖
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概要
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The present paper describes the results of experiments carried out with the aim of obtaining evidences other than the transovarial virus transmission, of multip1ication of the rice stripe virus in its insect vector, the sma11er brown planthopper Laodelphax striatellus Fa11en, and information about the possible site of virus multiplication and patho1ogical effects of the virus on the insect vector. Serial transfers of the rice stripe virus from insect to insect were made using microinjection method described by Maramorosch (1952). The virus was successfu11y carried in 4 serial passages in the sma11er brown planthopper. The final dilution of the inoclum used to inject the last group of the series is calculated to be at least 1.25×10 of the origina1 inoculum, while the virus derived from viru11ferous insects was shown to have a di1ution end-point between 10-2 and 10-8. Groups of planthoppers which had been injected with the juice from viru1iferous insects, were macerated 2, 5, 10 and 15 days after the injection, and the preparations were tested for infectivity by inoculating to virus free insects. The inocula prepared from the planthoppers 2 and 5 days after injection were found noninfective, in coincidence with the incubation period in the vector. A 1:10 dilution of the macerate from planthoppers 10 days after in jection and a l:50 dilution of those from planthoppers 15 days after injection proved infective. This suggests that the virus may have multip1ied in its vector at least fivehold during the period from 5th to 15th day after injection. B1ood and various organs from viru11ferous planthoppers were tested for the presence of virus by the injection method. Virus was recovered from eggs, male reproductive organs, and b1ood and fat body, while no virus was recovered from alimentary canal. Male planthoppers fol1owing 2 days of acquisition feeding on diseased rice plants were dissected to remove legs, wings, alimentary canal, Malpighian tubules and reproductive organ, and the remaining part was separated into head, thorax, and abdomen. These body parts were incubated for 13 days at 25-27℃ in hanging drops of a medium consisting of Takami's salt so1ution, D-glucose and extract from their respectivepart. The virus was not recovered from fresh materia1 of these parts, but was recovered from the abdominal part incubated in vitro for 13 days. Female and male reproductive organs excised from planthoppers which had fed for 3 days on diseased plants, were incubated for 12 days in vitro. From both organs virus was recovered after having been incubated for 12 days, but not before incubation. Cytological examinations of b1ood corpsucles, reproductive ce11s, salivary glands, a1imentary canal, dermis, and fat body of viru1iferous planthoppers showed no consistent, conspicuous changes in these tissues. Histochemical tests for hyaluronic acid, RNA, DNA, 1ipid, and phospho11pid, and phosphatase activity showed no distinct difference between viru1iferous and virus-free planthoppers. Relative amount of glycogen and of polysaccharides in the fat body and of mycetome, as determined by Bauer-Feulgen stain and by Li11ie method, appeared to be somewhat less in viru11ferous planthoppers than in virusfree ones.
- 日本植物病理学会の論文
- 1968-09-30
著者
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