灰色かび病菌の薬剤耐性菌培養ろ液によるジカルボキシイミド系薬剤耐性誘発検定

概要

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Induction of dicarboximide fungicide-resistance by pre-culturillg ill the filtrates of the field resistant strains (IHES-2 and CAES-4) of Botrytis cinerea was examined by two bioassay methods. In the protoplast bioassay method, the regeneration frequencies of the mycelial protoplasts of the sensitive strain (IPCR-1)pre-treated with IHES-2 and CAES-4 filtrates, respectively, were 2.7 al]d 2.4% on the medium containing the fungicide, which were about tenfold higher than that by pre-treatment with IPCR-l filtrate. In another method, IPCR-1 conidia were shaking-cultured in IHES-2 and CAES-4 filtrates for 3 days, respectively,and each of the conidia was harvested as a mycelial ball like structure (MBS). The MBSs pre-cultured in these filtrates formed the colonies about three times as many as those with IPCR-1 filtrate. From the two different bioassays, the induction of dicarboximide fungicide-resistance with the filtrates of the resistant strains was confirmed. Especially, MBS bioassay method may be a useful one for bioassays of filamentous fungi, in which it is hard to statistically detect the effects of physio1ogically active substances because of the morphogenic changes in the growth cycle.
日本植物病理学会の論文
1992-10-25