抗ウイルス剤の作用下に生じたタバコモザイクウイルスたんぱくのろ紙電気泳動

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The inhibitory effect of actidione and thiouracil against TMV production in tobacco leaf disks have been reported (Shimomura and Hirai, 1959). When these antiviral substances were applied to infected tissues and the amount of virus prcduced was determined by the cptical densities, the inhibitory effect was much less pronounced than that determined by the infectivity tests (local lesion method) (Shimomura, Nishikawa and Hirai, 1957). In the present report, the paper electrophoretic pattern of TMV protein prcduced in the presence of these antiviral substances are presented. The soluble protein of infected leaf tissues was obtained by O.6 saturated ammonium sulphate-precipitation and by its dissolution with the buffer of McIlvaine and Lillie (pH 7.0). These protein was run on filter paper for 3 hours using Toyoroshi No. C electrcphoretic apparatus and veronal buffer (pH 8.5, ionic strength 0.045). Potential of 400V was applied to give 0.5 mAlcm. The results were consistent with those obtained by Kanngiesser (1955) and both healthy protein and virus prctein were separately detected on filter paper. The virus protein, which was purified by repeated cycles of ammonium sulphate-precipitation and dissolution and again precipitation at the isoelectric point of the virus, remained almost at the origin even after 3 hours' run and showed only one peak. In contrast, the virus protein, produced in the presence of actidione or thiouracil, showed two distinct peaks. The problem whether these two fractions, shown by electrophoretic pattern, represent infective virus and noninfective virus is now under study, although existence of two types of virus in the infected tissues treated with antiviral substances has been proposed by Francki (1959-62).
日本植物病理学会の論文
1963-09-25

抗ウイルス剤の作用下に生じたタバコモザイクウイルスたんぱくのろ紙電気泳動

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