タバコ・モザイク・ウイルスによる Nicotiana gtinosalu 葉上の局部病斑の形成における表皮の役割

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When tobacco mosaic virus (TMV) was inoculated on the naked mesophyll tissues of Nicotiana glutinosa leaves from which the under epidermis had been removed, a few necrotic lesions were found on the stripped areas of the leaves. These lesions appeared a few days later than those on the leaves with epidermis and they were larger in size and less colored than normal lesions. It seemed that they were formed in close association with the intact upper epidermis which presumably became infected with TMV moved from the mesophyll tissues. When the epidermis-stripped areas of N. glutinosa leaves were inoculated with TMV and assayed for the virus content, it was found that TMV introduced into naked parenchymatous tissues could multiply to some extent. In the isolated epidermis removed from the under surface of leaves inoculated with TMV, the virus also multiplied provided the epidermis was incubated for 4 days floating on distilled water. No lesions, however, appeared on the epidermis. On the contrary, when the under epidermis of N. glutinosa leaves incubated at 22 C was removed 8-10 hr after inoculation of the epidermis, typical lesions same as those found on the epidermis-remaining areas were formed on the areas devoid of epidermis. When the upper epidermis was previously removed and the TMV-inoculated lower epidermis was removed about 10 hr after inoculation, lesions also appeared on the areas devoid of both epidermis. No lesions developed, however, on the naked mesophyll tissues from which the under epidermis had been removed 5 hr after inoculation. Bioassay of these areas revealed the presence of TMV in the stripped areas. It appears that interactions between epidermis and mesophyll tissues are needed for lesion formation even after TMV has reached to the mesophyll tissues. When inoculated leaves kept for some time at high temperature (28 C) were exposed to low temperature (20 C), lesions were formed. Since lesions were still found on the stripped areas when the epidermis was stripped immediately before the exposure of leaves to 20 C, it is suggested that low temperature is not always necessary for proceeding the interactions between epidermis and mesophyll tissues.
日本植物病理学会の論文
1977-04-25

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