花き類の根頭がんしゅ病に関する研究 : I. 病害の発生ならびに病原細菌の細菌学的性質

概要

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Crown gall diseases of chrysanthemum, marguerite and rose were found in Shizuoka prefecture during 1974-1977. The causal bacteria were isolated from these infected plants and were tested for their pathogenicities and bacteriological characteristics. Twenty bacterial isolates were identified as Agrobacterium tumefaciens (Smith & Townsend 1907) Conn 1942 based on their pathogenicities and 70 cultural and physiological characteristics. These isolates were separated into two biovars by the difference in the following 15 physiological characteristics : 3-ketolactose production, kovac's oxidase, NaCl tolerance, maximum growth temperature, litmus milk, growth facter requirement, utilization of tartrate, malonate, and citrate, acid production from raffinose and erythritol, nitrate respiration, H_2S production, arginine dihydrolase, and utilization of asparagine as a sole source of carbon and nitrogen. Six chrysanthemum, 6 marguerite and 4 rose isolates were classified into biovar 1, and other 4 rose isolates into biovar 2. DNA base composition was measured by the melting temperature (T_m) method. The T_m value of the isolates were in the range of 94.0-94.6 C, and the GC contents were in the range of 58.2-59.7%.
日本植物病理学会の論文
1984-04-25