カブモザイクウイルスの保存に及ぼす各種添加物の影響

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To establish a simple and practical method for preservation of turnip mosaic virus (TuMV), the effect of some additives was evaluated in connection with different preservation methods. (1) When an equal volume of glycerol was added to suspension of purified TuMV in 10mM potassium phosphate buffer (P. B.) pH7.0, the high infectivity was maintained for the period longer than 48 months under liquid conditon at-20C. (2) The infectivity of purified TuMV suspended in 10mM P. B. pH7.0 and crude extract from the infected turnip leaves in 50mM P. B. pH7.0 decreased gradually under frozen condition at -20C. The infectivity, however, was maintained at high level for long period by the addition of glycerol, sucrose or peptone. At -70C, the infectivity was maintained for long period without any additives. (3) By freeze-drying, the infectivity of TuMV in purified preparation decreased markedly, whereas that of crude extract from infected turnip leaves did not decrease. The virus aggregation was considered to be one of the mechanism of decrement of infectivity. The addition of peptone, sucrose or lactose prohibited the aggregation. To obtain the results of protective effect of additives in freeze-drying preservation as quick as possible, the preparations were preserved at higher temperature of 25C and 65C. At 25C, glycine and peptone showed protective effect, while at 65C, only peptone was effective. Glycine was effective for crude extract, while it was ineffective for the purified preparation. Freeze-dried TuMV was considerably stable at 4C and it was quite stable at a temperature lower than -20C, regardless of the kind of additives.
日本植物病理学会の論文
1983-04-25

カブモザイクウイルスの保存に及ぼす各種添加物の影響

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