A Lipase-inhibiting Protein from Lipoxygenase-deficient Soybean Seeds(Biochemistry & Molecular Biology)
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概要
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A lipase-inhibiting protein was isolated from lipoxygenase (LOX)-deficient soybean seeds. The molecular mass of the protein was 56.0-kDa and the N-terminal amino acid was blocked. The protein was identified by peptide mass fingerprinting in combination with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry. The masses of the lysyl endopeptidase-digested peptides of the 56.0-kDa inhibiting protein were almost identical to the calculated masses of the theoretically predicted lysyl endopeptidase-treated peptides of β-amylase from soybean seed. In a previous paper (Biosci. Biotechnol. Biochem., 62, 1498-1503, 1998), we reported that LOX-1, an isozyme of soybean seed LOX, inhibited hydrolysis of soybean oil by pancreatic lipase. Purified β-amylase also inhibited lipase activity, although the magnitude of inhibition was weaker than that by LOX-1. Thus, there are at least two lipase-inhibiting proteins, one is a LOX and the other is a β-amylase, in soybean seed.
- 社団法人日本農芸化学会の論文
- 2002-10-23
著者
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TANAKA Tamotsu
Department of Applied Biological Science, Fukuyama University
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SATOUCHI Kiyoshi
Department of Applied Biological Science, Fukuyama University
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MURAKAMI KAORU
Department of Applied Chemistry, Faculty of Enggineering, Kyushu Institute of Technology
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IWAMOTO Hiroyuki
Department of Food Science and Technology, Faculty of Engineering, Fukuyama University
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Murakami Kaoru
Department Of Applied Biological Science Fukuyama University
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Ishimoto Masao
Biological Laboratory Of Plant Biotechnology National Agricultural Research Center For Western Regio
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Tanaka Tamotsu
Department Of Applied Biological Science Fukuyama University
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KODAMA Yoshifumi
Department of Applied Biological Science, Fukuyama University
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Satouchi Kiyoshi
Department Of Applied Biological Science Fukuyama University
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Iwamoto Hiroyuki
Department Of Applied Biological Science Faculty Of Life Science And Biotechnology Fukuyama Universi
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Iwamoto Hiroyuki
Department Of Applied Biological Science Fukuyama University
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Kodama Yoshifumi
Department Of Applied Biological Science Fukuyama University
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