Prolyl Endopeptidase Inhibitory Activity of Peptides in the Repeated Sequence of Various Proline-Rich Proteins
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概要
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Peptides present in the repeating region in proline-rich proteins such as corn γ-zein, soybean cell wall protein (SbPRP1), carrot 33-kDa protein, collagen and mouse salivary gland protein were chemically synthesized. The prolyl endopeptidase (PEP, from Flavobacterium meningosepticum or bovine brain) inhibitory activity of these peptides was investigated. γ-Zein-related synthetic peptides such as His-Leu-Pro-Pro-Pro-Val and His-Leu-Pro-Pro-Pro-Val-His-Leu-Pro-Pro-Pro-Val inhibited PEP from both bacterial and meammalian sources.The IC_<50> values of these peptides for F. meningosepticum PEP were 80 μM and 30 μM, respectively. Soybean cel l wall protein-related synthecic peptides, Lys-Pro-Pro-Val and Lys-Pro-Pro-Ile also inhibited PEP to some degree. However, Pro-Pro-Pro-Pro-Gly-Gly-Pro-Glin-Pro-Arg-Pro-Pro-Gln-Gly (synthetic peptide fragment of salivary gland protein) and Gly-Pro-Hyp-Gly-Pro-Ala (fragment of collagen) showed hardly any inhibition.Therefore, we hydrolyzed γ-zein with sobtilisin (Carlsberg) and confirmed the liberation of the native hexapeptide, His-Leu-Pro-Pro-Pro-Val.
- 社団法人日本生物工学会の論文
- 1992-09-25
著者
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Maruyama Susumu
Fermentation Research Institute
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Maruyama Susumu
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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Maruyama Susumu
National Institute Of Bioscience And Human Technology Agency Of Industrial Science And Technology
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MIYOSHI Shinsuke
Research and Development Center, Showa Sangyo Co., Ltd.
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TANAKA Hideoki
Fermentation Research Institute
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Tanaka Hideoki
National Institute Of Bioscience And Human-technology Agency Of Industrial Science And Technology
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OSA TAKASUMI
Saitama Institute of Technology
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Miyoshi S
Showa Sangyo Co. Ltd. Ibaraki Prefecture Jpn
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