Inhibition of Vero Cell Cytotoxic Activity in Escherichia coli O157 : H7 Lysates by Globotriaosylceramide, Gb3,from Bovine Milk
スポンサーリンク
概要
- 論文の詳細を見る
In order to clarify the presence and verotoxin (VT) inhibitory activity of globotriaosylceramide (Gb3) in bovine milk, we analyzed neutral glycosphingolipids (GSLs) from bovine milk and investigated the inhibitory effect of bovine milk Gb3 on the cytotoxicity of TV2. Five species of neutral GSLs, designated as N-1,N-2,N-3,N-4,and N-5,were separated on thin-layer chromatography (TLC). N-1,N-2,and N-3 showed the same mobility as glucosylceramide, lactosylceramide, and Gb3 on the TLS plate, respectively. N-4 and N-5 GSLs migrated below globoside on the TLC plate. N-3 GSL having the same TLC mobility as Gb3 from bovine milk was immunologically identified as Gb3 by monoclonal antibody against Gb3,anti-CD77 monoclonal antibody. Furthermore, the effect of bovine milk Gb3 on VT2-induced cytotoxicity was investigated. We found that treatment of VT2 with bovine milk Gb3 can reduce the cytotoxic effect of VT2.
- 社団法人日本農芸化学会の論文
- 2001-02-23
著者
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INOUE Kaoru
Department of Health Sciences, Hokkaido University School of Medicine
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YOKOTA Kenji
Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry
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OGUMA Keiji
Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry
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ISOGAI Emiko
Department of Hygiene, Health Sciences University of Hokkaido
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Oguma Keiji
Department Of Bacteriology Okayama University Graduate School Of Medicine And Dentistry
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Inoue Kaoru
Department Of Health Sciences Hokkaido University School Of Medicine
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Yokota K
Department Of Bacteriology Okayama University Graduate School Of Medicine And Dentistry
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Yokota K
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Yokota Kenji
Department Of Bacteriology Okayama University Graduate School Of Medicine And Dentistry
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KUSHI Yasunori
Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental university
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Kodama Hiroshi
Laboratory Of Veterinary Immunology Division Of Veterinary Science Graduate School Of Agriculture An
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Oguma K
Okayama Univ. Graduate School Of Medicine And Dentistry Okayama Jpn
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Yokota Kenji
Okayama Univ. Graduate School Of Medicine And Dentistry Okayama Jpn
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Inoue K
Department Of Bacteriology Okayama University Medical School
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Isogai E
Department Of Preventive Dentistry Health Science University Of Hokkaido
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Kushi Yasunori
Department Of Biochemistry Faculty Of Medicine Tokyo Medical And Dental University
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Kodama Hiroshi
Laboratory Of Veterinary Immunology Course Of Veterinary Science Graduate School Of Life And Environ
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WATARAI Shinobu
Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka
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Watarai S
Laboratory Of Veterinary Immunology Division Of Veterinary Science Graduate School Of Agriculture An
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Tajima Shigeru
Retrovirus Research Unit Riken
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NAKA Katsumi
Department of Cell Chemistry, Institute of Cellular and Molecular Biology, Okayama University Medica
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Watarai Shinobu
Laboratory Of Veterinary Immunology Depertment Of Veterinary Science College Of Agriculture Osaka Pr
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Naka Katsumi
Department Of Cell Chemistry Institute Of Cellular And Molecular Biology Okayama University Medical
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Inoue Kaoru
Department Of Bacteriology Okayama University Medical School
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Yokota Kenji
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic
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Oguma Keiji
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutic
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Yokota Kenji
Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agric
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Inoue Kaoru
Department Of Anatomy Hokkaido University School Of Medicine
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KUSHI Yasunori
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine
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