Cloning, Monoclonal Antibody Production, and Bodily Distribution Pattern of a Bovine Lipocalin
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概要
- 論文の詳細を見る
A bovine lipocalin, previously identified as a putative odorant-binding protein in bovine colostrum (bcOBP), was cloned and expressed, and its monoclonal antibody was established. bcOBP was constantly secreted into milk on day of parturition until at least 10 d postpartum at a concentration of 181±39 μg/L. Besides milk, bcOBP occurred in the nasal mucus, saliva, amniotic fluid, vaginal discharge, and blood plasma. Despite its low concentration, the distribution pattern and the finding that bcOBP harbored a characteristic sequence motif, CxxxC, which is conserved among insect and mammal pheromone binding proteins, suggest that bcOBP functions as a pheromone carrier. The presence of bcOBP in the plasma at varied concentrations depending on the lactation period does not exclude the possibility that bcOBP is secreted into milk from the blood. Cross-reactivity of the monoclonal antibody indicated presence of proteins homologous to bcOBP in the colostrum of farm animals of Cetartiodactyla.
- Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
著者
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Suzuki Takumi
Department Of Electronics Tohoku Institute Of Technology
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Kida Katsuya
Field Center Of Animal Science And Agriculture Obihiro University Of Agriculture And Veterinary Medi
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Kushi Yasunori
Department Of Biochemistry Faculty Of Medicine Tokyo Medical And Dental University
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Senda Akitsugu
Graduate School Of Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Senda Akitsugu
Department Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Urashima Tadasu
Department Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Fukuda Kenji
Department Of Animal And Food Hygiene Obihiro University Of Agriculture And Veterinary Medicine
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Yanagida Mayumi
Department Of Agriculture And Life Science Obihiro University Of Agriculture And Veterinary Medicine
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JAPARIDZE Tamar
Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
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NOZAKI Hirofumi
Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology
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GANZORIG Khuukhenbaatar
State Central Veterinary Laboratory
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BRUCKMAIER Rupert
Veterinary Physiology, Vetsuisse Faculty, University of Bern
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