A Competitive Enzyme-Linked Immunosorbent Assay-like Method for the Measurement of Urinary Hyaluronan
スポンサーリンク
概要
- 論文の詳細を見る
A highly sensitive method for measurement of urinary hyaluronan with a minimum molecular mass of 2,000 Da was developed without using HPLC or radioisotopes. This competitive enzyme-linked immunosorbent assay-like method, used competitive binding of free hyaluronan in the sample and biotin-labeled standard hyaluronan to hyaluronan binding protein in solid phase. A total of 150 healthy individuals from both sexes at ages from 0 to 100 years was examined by the established method. Hyaluronan of 384±80 ng/mg creatinine (mean±SD) was constantly excreted into urine of 24-40-year-old healthy adults. The urinary hyaluronan levels were significantly higher before age 1 (p<0.001) and rather high after 90 years compared to the other groups. The average molecular weight of urinary hyaluronan (5,500 Da) was constant through all generations. Sex difference of urinary hyaluronan was not observed both quantitatively or qualitatively.
- 社団法人日本農芸化学会の論文
- 1999-05-23
著者
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Fujita Hiroshi
Department Of Intelligent Image Information Division Of Regeneration And Advanced Medical Sciences G
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GOTO Makoto
Department of Rheumatology, Tokyo Metropolitan Otsuka Hospital
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Fujita H
Department Of Intelligent Image Information Division Of Regeneration And Advanced Medical Sciences G
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MAEDA Hiroshi
Center for Glycosaminoglycan Analysis, Seikagaku Kogyo Co.
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FUJITA Hiroshi
Center for Glycosaminoglycan Analysis, Seikagaku Kogyo Co.
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SAKURA Yoshiyuki
Center for Glycosaminoglycan Analysis, Seikagaku Kogyo Co.
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MIYAZAKI Kyosuke
Center for Glycosaminoglycan Analysis, Seikagaku Kogyo Co.
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Fujita Hiroshi
Graduate School Of Medicine Gifu University
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Sakura Yoshiyuki
Center For Glycosaminoglycan Analysis Seikagaku Kogyo Co.
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Miyazaki Kyosuke
Center For Glycosaminoglycan Analysis Seikagaku Kogyo Co.
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Goto Makoto
Department Of Electronic Engineering Gunma University
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Goto Makoto
Department Of Rheumatology Tokyo Metropolitan Otsuka Hospital
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Goto Makoto
Department Of Chemistry Tohoku University & Crest
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Maeda Hiroshi
Center For Glycosaminoglycan Analysis Seikagaku Kogyo Co.
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GOTO Makoto
Department of Aerospace Engineering, Nagoya University
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