Solid-state Spin Trapping of the Hydroxyl Radical Formed by Gamma-irradiation in Ice and the Scavenging Effect of Sodium L-Ascorbate
スポンサーリンク
概要
- 論文の詳細を見る
Spin trapping of the hydroxyl (OH) radical formed by γ-irradiation in ice was performed by using 5,5-dimethyl pyrroline N-oxide (DMPO) as a trapping agent. A frozen aqueous solution of DMPO was γ-irradiated at -70℃ for three days, and ESR spectra were then measured at room temperature as a function of time after melting. A strong four-line absorption characteristic of the spin adduct (DMPO-OH) was observed and the intensity decreased with time, while no signal was observed when γ-irradiation was carried out at room temperature. The intensity of DMPO-OH increased linearly with radiation dose, but it was not changed by the standing time at -70℃ after γ-irradiation, suggesting that the OH radical formed by γ-irradiation in ice was trapped by DMPO in the frozen state, DMPO-OH being stable for more than three days in contrast to the case at room temperature. The quantity of DMPO-OH was decreased in the presence of sodium L-ascorbate. The analysis of the intensity change shows that this solid-state spin trapping technique is useful to measure separately the OH radical scavenging and DMPO-OH scavenging abilities of antioxidants.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
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YOSHIOKA Hisashi
Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
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YOSHIOKA Hisashi
Institute for Environmental Sciences, University of Shizuoka
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Hasegawa Kunihiko
Department Of System Engineering Of Materials And Life Science Faculty Of Engineering Toyama Univers
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Yoshioka H
Univ. Shizuoka Shizuoka Jpn
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YOSHIOKA Hiroe
Radiochemical Research Laboratory, Faculty of Science, Shizuoka University
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HASEGAWA Kunihiko
Radiochemistry Research Laboratory, Faculty of Science, Shizuoka University
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Yoshioka Hisashi
Institute For Environmental Science University Of Shizuoka
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Hasegawa Kunihiko
Department Of Chemistry Faculty Of Science And Radiochemistry Research Laboratory Shizuoka Universit
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Hasegawa Kunihiko
Radiochemistry Research Laboratory Faculty Of Science Shizuoka University
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Yoshioka Hisashi
Graduate School Of Nutritional And Environmental Sciences And School Of Pharmaceutical Sciences Univ
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Hasegawa Kimihiko
Radiochemical Research Laboratory, Faculty of Science, Shizuoka University
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