Kinetic Analysis of the Effect of (-)-Epigallocatechin Gallate on the DNA Scission Induced by Fe(II)(Food & Nutrition Science)

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概要

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• The DNA strand scission induced by Fe(II) in a citrate buffer solution and the effect of (-)-epigallocatechin gallate (EGCg) were kinetically analyzed. The rate of consumption of dissolved oxygen by Fe(II) in each of these solutions was measured and paralleled that DNA scission. Coordinated EGCg accelerated these reactions. Curves of the time-course characteristics of DNA scission were simulated by using the rate constant of oxygen consumption and by assuming that scission with the hydroxyl radical (OH), which was formed from the dissolved oxygen, proceeded competitively with the scavenging of OH by citrate, Cl^- ions and EGCg added. Free EGCg acted as a DNA scission inhibitor to scavenge OH, in contrast to the case of the coordinated one. This analysis is useful for estimating the rate constant of the reaction between an antioxidant and OH, and might provide a new method for measuring the OH-scavenging activity.

• 2002-04-23

著者

• YOSHIOKA Hisashi

  Institute for Environmental Sciences, University of Shizuoka

• Yoshioka H

  Univ. Shizuoka Shizuoka Jpn

• YOSHIOKA Hiroe

  Radiochemical Research Laboratory, Faculty of Science, Shizuoka University

• Yoshinaga Koichi

  Faculty Of Science Shizuoka University

• OHASHI Yasunori

  Radiochemistry Research Laboratory, Shizuoka University

• Ohashi Y

  National Food Res. Inst. Ibaraki Jpn

• Yoshioka Hisashi

  Institute For Environmental Science University Of Shizuoka

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