Cloning of Stress Tolerance Gene in Torulaspora delbrueckii No. 3110
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概要
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A cryptic plasmid (pSRY1) was found in Torulaspora delbrueckii No. 3110. A plasmid vector (pSRY21) was constructed by ligating it to pPR1-RIM-C ORF with a cycloheximide resistance gene (RIM-C). The transformation efficiency of the strain No. 3110 with pSRY21 by the protoplast-PEG method was increased by 3000-fold by treatment of the protoplasts with polylysine [poly(ε-L-lysine)]. Previously we have reported a mutant of No. 3110, T1, which can neither assimilate nor accumulate trehalose. The mutant was salt- and temperature-sensitive. We cloned a DNA fragment from No. 3110, which, when introduced, complemented these mutant characters of T1. These results suggested an important role of metabolism of trehalose for the stress tolerance.
- 社団法人日本農芸化学会の論文
- 1996-10-23
著者
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Nakata Kuniho
Central Research Laboratories Mercian Corporation
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Okamura Kazuhiko
Central Research Laboratories Mercian Corporation
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Okamura Kazuhiko
Central Research Laboratories Marcian Corp.
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Okamura Kazuhiko
Central Research Laboratories Mercian Corporation:japan Bioindustry Association
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