58. イネの細胞質Ca^<2+>濃度の非破壊モニタリング系の確立とCa^<2+>チャネルを介したCa^<2+>動員制御機構の解析

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Changes in cytosolic free calcium ion concentration ([Ca^<2+>]_<cyt>) triggered by environmental stimuli play important roles as a second messenger in plants. We here established a noninvasive monitoring system for [Ca^<2+>]_<cyt> in rice cells and plants expressing apoaequorin. [Ca^<2+>]_<cyt> changes in response to various stimuli including osmotic stress and pathogen-associated molecular patterns (elicitors) were monitored in both cultured cells and plants both in the wild type and a knockout mutant of a putative voltage-dene-pendent Ca^<2+> channel, OsTPC1 (Plant Cell Physiol. (2004) 45 : 693-; Plant J. (2005) 42 : 798-). Cytosolic Ca^<2+> dynamics induced by various environmental stimuli will be presented and possible molecular mechanisms and physiological significance of [Ca^<2+>]_<cyt> changes in various signal transduction pathways will be discussed.
2005-10-13