タバコおよびイネの懸濁培養細胞における温度変化応答性の急激なカルシウムの取り込み

概要

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The temperature is one of the key environmental factors surrounding the living organisms in nature. In plant cells, transient increase in cytosolic Ca^<2+> concentration ([Ca^<2+>]_c) occurs as a response to an acute temperature change. Such a rise in [Ca^<2+>]_c reportedly leads to cold acclimatization. Here, we examined the effect of the temperature- shifts from ambient to the lower or higher temperatures, on induction of [Ca^<2+>]_c increases, using suspension-cultured plant cell lines expressing aequorin, a luminescent [Ca^<2+>]_c-reporter. The cultures used here include three tobacco (Nicotiana tabacum L.) cell lines, namely BY-2, Bel-W3 (O_3-sensitive) and Bel-B (O_3-tolerant), and rice (Oryza sativa L., cv. Nipponbare) AQ7 cells. Generally, rice cells and tobacco cells behaved similarly. The levels of [Ca^<2+>]_c spikes induced by cold shock were much greater than those induced by heat shock in all cell lines tested. Effects of inhibitors suggested that the cold shock-induced [Ca^<2+>]_c spike requires the uptake of extracellular Ca^<2+> via plasma membrane Ca^<2+> channels, while the heat shock response might be due to channel-independent leakages of extracellular and organelle Ca^<2+> into cytoplasm.
低温生物工学会の論文
2006-12-30