マイクロフローチャンバによる細胞分析

概要

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Effect of the flow characteristics in a newly developed micro-machined sheath flow chamber on the accuracy of cytometric analysis was investigated. The chamber was formed by laminating three 100-μm-thick photo-etched metal plates. Sample fluid containing cells, which enters the chamber via an axial specimen nozzle, is enveloped by a buffering sheath-fluid and transported to a capillary channel. Microscopic observation and three-dimensional viscous flow analysis predict that the dispersion of cells in the capillary channel is favorably reduced when the Reynolds number is around 500〜750, which is value associated with low deviation of the analytical results. The five classifications of human white blood ceils were determined, and this verified that change of the deviation depends on the Reynolds number. The results confirm Reynolds number of around 500〜750 is a appropriate for good classification.
社団法人日本機械学会の論文
1999-01-25