Participation of Thrombospondin-1 in the Activation of Latent Transforming Growth Factor-β in Malignant Glioma Cells

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概要

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• Malignant glioma cells secrete transforming growth factor-β (TGF-β) and can activate latent TGF-β. However, the mechanism of the latent TGF-β activation has not yet been determined. This study examined whether thrombospondin-1 (TSP-1) secreted by malignant glioma cell lines participates in the activation of latent TGF-β secreted by the glioma cells. Western blot analysis revealed that TSP-1 was present in both the cell lysates and the culture supernatants of all three malignant glioma cell lines (T98G, A172, and U251). A biossay for TGF-β activity revealed that all malignant glioma cell lines used in this study could activate latent TGF-β by themselves. Latent TGF-β1 activation, evaluated by enzyme-linked immunosorbent assay, was inhibited by more than 50% by the addition of neutralizing anti-TSP-1 monoclonal antibody or anti-TSP-1 polyclonal antibody. These results indicate that TSP-1 has a predominant role in the activation of latent TGF-β in malignant glioma cells.

• 日本脳神経外科学会の論文
• 2001-05-15

著者

• KAWATAKI Tomoyuki

  Department of Neurosurgery, University of Yamanashi, Faculty of Medicine

• SASAKI Atsushi

  Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, Univers

• Sasaki Atsushi

  Department Of Applied Pharmacology Graduate School Of Medicine And Pharmaceutical Sciences Universit

• NAGANUMA Hirofumi

  Department of Neurosurgery, University of Yamanashi, Faculty of Medicine

• SATOH Eiji

  Department of Neurosurgery, University of Yamanashi Faculty of Medicine

• AMAGASAKI Kenichi

  Department of Neurosurgery, University of Yamanashi Faculty of Medicine

• NUKUI Hideaki

  Department of Neurosurgery, University of Yamanashi Faculty of Medicine

• Kawataki Tomoyuki

  Department Of Neurosurgery Yamanashi Medical University

• Kawataki Tomoyuki

  Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine

• Satoh Eiji

  Department Of Cardiology Sendai City Hospital

• Naganuma H

  Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine

• Naganuma Hirofumi

  山梨大学 医学部脳神経外科学

• Naganuma Hirofumi

  Department Of Neurosurgery National Hospital Organization Kofu Hospital

• Nukui H

  Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine

• Nukui Hideaki

  Department Of Neurosurgery And Radiology Yamanashi Medical University And Department Of Radiology To

• Naganuma H

  Department Of Neurosurgery Yamanashi Medical University

• Nukui Hideaki

  Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine

• Amagasaki Kenichi

  Department Of Neurosurgery Yamanashi Medical University

• Amagasaki Kenichi

  Department Of Neurosurgery Kanoiwa General Hospital

• Sasaki Atsushi

  Department Of Neurosurgery Yamanashi Medical University

• Naganuma Hirofumi

  Department of Neurosurgery, University of Yamanashi Faculty of Medicine

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