Improved Bioassay for the Detection of Transforming Growth Factor-β1 and β2 in Malignant Gliomas
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概要
- 論文の詳細を見る
Growth inhibition assays using radioisotope or dye are used to detect transforming growth factor-β (TGF-β). Here, we describe a modified bioassay using crystal violet for the quantitative detection of TGF-β1 and TGF-β2. The procedure is based on staining Mv1Lu mink lung epithelial cells with crystal violet, followed by measurement of the absorbance at 570nm in individual wells of a 96-well microtiter plate. The number of Mv1Lu cells correlated with the eluted dye intensity. The sensitivity of the bioassay to recombinant TGF-β1 and TGF-β2 increased approximately twofold by using only 500 Mv1Lu cells in microtiter wells. The bioassay was used to measure TGF-β activity in the culture supernatant from glioblastoma cells. Culture supernatants were untreated or acid-activated to quantify the active or total TGF-β, and neutralized with anti-TGF-β1 and/or anti-TGF-β2 antibody to measure the activity. Both TGF-β1 and TGF-β2 were detected in the untreated and acid-activated supernatants, and the amounts were calculated by extrapolating from the known recombinant TGF-β1 or TGF-β2 dilution curve. Our results show that the modified bioassay using crystal violet can measure the levels of TGF-β1 and TCF-β2 in culture supernatants from malignant glioma cells.
- 日本脳神経外科学会の論文
- 1994-03-15
著者
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SASAKI Atsushi
Department of Applied Pharmacology, Graduate School of Medicine and Pharmaceutical Sciences, Univers
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Tasaka K
Department Of Parasitology And Immunology Yamanashi Medical University
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Tasaka Kachio
Department Of Parasitology And Immunology Yamanashi Medical University
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Sasaki Atsushi
Department Of Applied Pharmacology Graduate School Of Medicine And Pharmaceutical Sciences Universit
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NAGANUMA Hirofumi
Department of Neurosurgery, University of Yamanashi, Faculty of Medicine
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SATOH Eiji
Department of Neurosurgery, University of Yamanashi Faculty of Medicine
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NUKUI Hideaki
Department of Neurosurgery, University of Yamanashi Faculty of Medicine
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Satoh Eiji
Department Of Cardiology Sendai City Hospital
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Naganuma H
Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine
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Naganuma Hirofumi
山梨大学 医学部脳神経外科学
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Naganuma Hirofumi
Department Of Neurosurgery National Hospital Organization Kofu Hospital
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Nukui H
Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine
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Nukui Hideaki
Department Of Neurosurgery And Radiology Yamanashi Medical University And Department Of Radiology To
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Sakihama Toshiko
Department of Parasitology and Immunology, Yamanashi Medical College
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Sakihama T
Department Of Parasitology & Immunology Yamanashi Medical College
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Sakihama Toshiko
Department Of Parasitology And Immunology Yamanashi Medical College
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Sakihama Toshiko
Department Of Experimental Pathology Institute For Frontier Medical Sciences
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Naganuma H
Department Of Neurosurgery Yamanashi Medical University
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Nukui Hideaki
Department Of Neurosurgery University Of Yamanashi Faculty Of Medicine
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Sasaki Atsushi
Department Of Neurosurgery Yamanashi Medical University
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Tasaka Kachio
Department Of Immunology Faculty Of Medicine University Of Yamanashi
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Naganuma Hirofumi
Department of Neurosurgery, University of Yamanashi Faculty of Medicine
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TASAKA Kachio
Department of Parasitology and Immunology, Yamanashi Medical College
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