胆道閉鎖症における血清3β-Hydroxy-5-Cholenoic-Acidの測定意義と血清硫酸並びに血清グルクロン酸抱合型胆汁酸についての検討

概要

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3-hydroxy-5-cholenoic acid which is one of monohydroxy bile acid with hepatotoxic effects, has been considered the product of an altered pathway of biosynthesis of bile acid in the liver. In the serum of cholestatic patients, it increases as a form of either sulfate-conjugated bile acid or glucuronide-conjugated bile acid. Bile acids in serum patients with biliary atresia and neonatal hepatitis were measured by high performance liquid chromatography (HPLC) combined with immobilized enzyme in colum form and new analytical method using sulfatase and β-glucuronidase. In preoperative biliary atresia group and neonatal hepatitis group, sulfate-conjugated bile acid was more dominant than glucuronide-conjugated bile acid. However, in postoperative biliary atresia group with persistent jaundice, consentration of glucuronide-conjugated bile acid was as much as that of sulfate-conjugated bile acid. Our studies suggested that glucuronide-conjugation might be related to development of UDP-glucuronyl-transferase activity and might play an important role in excreting abnormal bile acid into urine as well as sulfate-conjugation.
日本小児外科学会の論文
1988-06-20