DNAを用いた微小粒子のナノ位置決め(第1報) : 基本原理の提案と実験的検証

概要

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A novel nano-mechanism assemble technique using DNA strand and chemically modified gold nano-particles has been proposed. Four types of DNA short single strand have been synthesized and hybridized to form a unit-block of the nano-mechanism. One of the DNA strand is labeled by gold nano-particle so that each unit-block of the DNA works as a carrier and positioner of the particle. After ligating the unit-blocks by ligase, a long strand of DNA chain with periodic nano-particle can be assembled. A positioning resolution of 0.332nm, which is the helical pitch of the nuculic acid in solution, can be obtained in this technique. An experiment has been performed using three types of 33bp(base-pair) DNA and a 99bp DNA to form the unit-block, as well as avidin-biotin labeling technique for the nano-particle coupler. AFM micrographs show serial nano-particle chains with pitches of 31.3-32.8nm, which is almost the same as the theoretical values. This result indicates the usefulness of the proposed technique for nano-mechanism assembling.
公益社団法人精密工学会の論文
2004-06-05