EFFECT OF APPLICATION OF GLUCOSE, CELLULOSE, AND RICE STRAW ON NITROGEN FIXATION (ACETYLENE REDUCTION AND SOIL-NITROGEN COMPONENTS) IN ANAEROBIC SOIL

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The use of the plastic net bag method for the measurement of the decomposition rate of rice straw in the reduced layer enabled to demonstrate that the organic matter of rice straw consisted of at least two components, one rapidly decomposable (half-life, 17 days) and the other slowly decomposable (half-life, 58 days). Stimulation by rice straw of N_2-fixation under anaerobic (under N_2, gas) conditions was compared with that by cellulose and glucose. The peak of acetylene reduction activity (ARA) in the soil to which glucose had been added occurred on the 1st day of incubation. When cellulose or straw was added at 1%, the ARA peak occurred after 3 to 4 days. Peak height induced by cellulose was 3 times larger than that by straw. After 2 weeks of incubation, the activity was negligible. Addition of glucose and cellulose increased the number of colonies of anaerobic bacteria grown on nitrogen-free medium. The levels of hydrolyzable-N and α-amino acid-N increased by glucose or cellulose addition, presumably due to N_2 incorporation. These findings suggest the existence of a linkage between anaerobic cellulolytic bacteria and anaerobic N_2-fixing bacteria during the decomposition of cellulose or straw.
社団法人日本土壌肥料学会の論文