Metabolic conversion of N-methyl carbon of γ-glutamylmethylamide to caffeine in tea plants

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Tea seedlings were treated with ^<14>C-methylamine to cause synthesis of ^<14>C-γ-glutamylmethylamide (N-methyl-^<14>C). The metabolic conversion of γ-glutamylmethylamide was studied by tracing ^<14>C. ^<14>C-γ-Glutamylmethylamide (N-methyl-^<14>C) translocated from roots and cotyledons to shoots of tea seedlings, was converted almost entirely into caffeine. Conversion was greater in light-exposed samples. For those grown in the dark, the converted amount did not correspond to the total caffeine produced. More ^<14>C-γ-glutamylmethylamide was present in stems than in leaves, but with ^<14>C-caffeine, the opposite was found. When ^<14>C-γ-glutamylmethylamide or ^<14>C-methylamine was applied to leaf disks, ^<14>C-caffeine was biosynthesized from both substances.
日本植物生理学会の論文