Isolation of an Endophytic Diazotroph, Klebsiella oxytoca, from Sweet Potato Stems in Japan
スポンサーリンク
概要
- 論文の詳細を見る
The population density of endophytic bacteria in the stem of field-grown sweet potato cultivars (Beniotome [BO], Koganesengan [KS], and Shiroyutaka [SY]) in Miyakonojo, Miyazaki, Japan, ranged from 10^2 to 10^4 cells g^-1 fresh weight sample using a semi-solid nitrogen-free medium. Eleven strains were isolated from the stems and two isolates, BO-1 and BO-5, showed a positive reaction in the acetylene reduction activity (ARA) test. BO-1 and BO-5 were isolated from cv. Beniotome in September 1999 and 2000, respectively. Morpho-physiological characterization of these isolates revealed that BO-1 and BO-5 showed a similar colony color in potato sucrose agar slants, produced bubbles in a modified semi-solid medium, acidified the medium, and displayed similar characteristics using the API 20NE rapid diagnostic kit. Partial sequence analysis of 16S rRNA from BO-1 revealed a 100% similarity (491 bp) to that of Klebsiella oxytoca. The other 9 isolates showed a negative reaction in the ARA test, slightly acidified or did not acidify the medium. Partial sequence analysis of the 16S rRNA revealed that the isolate SY-2 corresponded to Methylobacterium sp. (99.3% similarity for 1,241 bp), BO-3 to Pantoea agglomerans (99.1% similarity for 469 bp), and BO-8 to Sphingomonas sanguinis (98.8% similarity for 419 bp).
著者
-
Nakatani Makoto
Department of Cardiology, Himeji Cardiovascular Center
-
Adachi Katsuki
Department Of Agricultural Chemistry Kyoto University
-
Adachi Katsuki
Department Of Upland Farming Research National Agricultural Research Center For Kyushu Okinawa Regio
-
NAKATANI Makoto
National Institute of Crop Science
-
Nakatani M
National Institute Of Crop Science
-
Mochida Hideyuki
Department of Upland Farming Research, National Agricultural Research Center for Kyushu Okinawa Regi
-
Mochida Hideyuki
Department Of Upland Farming Research National Agricultural Research Center For Kyushu Okinawa Regio
関連論文
- Population of Diazotrophic Endophytes in Stem Apoplast Solution of Sugarcane and Related Grass Species in Tanegashima, Japan
- USEFULNESS AND LIMITATION OF CORONARY ANGIOPLASTY IN ACUTE MYOCARDIAL INFARCTION : IMPORTANCE OF RESIDUAL FLOW : PTCA, Thrombosis : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Extraction of Antioxidants in Sweetpotato Waste Powder with Supercritical Carbon Dioxide
- Isolation of an Endophytic Diazotroph, Klebsiella oxytoca, from Sweet Potato Stems in Japan
- Effect of Ammonium Ion and Temperature on Anthocyanin Composition in Sweet Potato Cell Suspension Culture
- Genotypic Diversity of Anthocyanin Content and Composition in Purple-Fleshed Sweet potato (Ipomoea batatas (L.) Lam)
- Identification of a Novel SINE-like Sequence in Retrotransposon Fragments of Sweet Potato
- Studies on storage root formation process of sweet potato using storage root formation suppressed mutant
- Changes in Anthocyanin Content and Composition of Developing Storage Root of Purple-Fleshed Sweet Potato (Ipomoea batatas (L.)Lam)
- EFFECT OF APPLICATION OF GLUCOSE, CELLULOSE, AND RICE STRAW ON NITROGEN FIXATION (ACETYLENE REDUCTION AND SOIL-NITROGEN COMPONENTS) IN ANAEROBIC SOIL
- Development and Characterization of Microsatellite Markers in Sweetpotato
- Genetic Analysis of Sweetpotato and Wild Relatives using Inter-simple Sequence Repeats (ISSRs)
- EFFECT OF THE APPLICATION OF LIGNIN AND/OR CHITIN TO SOIL INOCULATED WITH Fusarium oxysporum ON THE VARIATION OF SOIL MICROFLORA AND PLANT GROWTH
- Effects of percutaneous transluminal coronary angioplasty on segmental left ventricular function in patients with acute myocardial infarction.
- Studies on the Breeding for Improving Starch Properties in Sweet Potato