微分スペクトル法による作物中のオキシン銅の残留分析

概要

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Application of derivative spectrophotometry to residue analysis of copper 8-quinolinolate in crops was investigated. The result showed that the derivative spectrophotometry was more suitable than colorimetry, atomic absorption spectrometry and high performance liquid chromatography which were already published. Copper 8-quinolinolate was extracted with acidic methanol from crops. After addition of 1% sodium chloride solution to the methanol extract, the mixture was washed with n-hexane. After neutralizing, the aqueous methanol solution was extracted with dichloromethane. Finally, the pesticide in the dichloromethane solution was re-extracted with 1 N hydrochloric acid. Fourth derivative spectrum of the acidic solution was recorded by a Hitachi spectrophotometer type 330. The amount of copper 8-quinolinolate was proportional to the difference absorbance (ΔA=A_<252>-A_<261>) within the range of 0-0.3 ppm. The detectable limit of copper 8-quinolinolate was 0.04 ppm when 50 g of sample was used. Average recovery was about 95%.
日本農薬学会の論文
1982-11-20