バイオケモプリベンション : がん予防への新コンセプト : 第28回大会シンポジウム : I.がん化学予防の分子生物学的アプローチ

概要

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Our aim of the study on anti-carcinogenic agents is the development of "designer foods" or "functional foods" for cancer prevention ; i.e., the creation of new type of foods, which are fortified the cancer preventive function. Food-originated anti-carcinogenic agents seem to be especially useful for this purpose. As the prototype experiment to accomplish this project, we established mammalian cells synthesizing phytoene, an anti-carcinogenic carotenoid, by introduction of the crtB gene, phytoene synthase gene. Expression plasmid, pCAcrtB, was constructed to transfer the crtB gene to mammalian cells, and transfected to NlH3T3 cells and HeLa cells by electroporation and/or lipofection. Phytoene was detected in these cells. Recently, we have also succeeded to express limonene synthase gene in mammalian cells. Since limonene has been suggested to be a possible anti-carcinogenic phytochemical, we will continue this study and lead it to future projects for the development of valuable functional foods.
日本環境変異原学会の論文
2000-07-31