Mechanical Fluid Flow Enhances Proliferation by A549 Pulmonary Epithelial Cells
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概要
- 論文の詳細を見る
Background & Aims: Pulmonary epithelial cells line the human respiratory tract and are covered with respiratory tract mucus. Fluid flow affects them, because they are exposed to the shearing force of the mucus slab. In this study, we investigated the effect of mechanical fluid flow on the proliferation by A549 pulmonary epithelial cells. Methods : We used a Mild mixer to produce fluid flow by rotating the stand. Results : Mechanical fluid flow enhanced proliferation by A549 cells, and the stimulation of cell proliferation was unaffected by either the competitive inhibitor of nitric oxide synthase N^G-monomethyl-L-arginine or by sodium nitroprusside, which spontaneously generates nitric oxide. However, it was reduced by the guanylate cyclase inhibitor methylene blue. Conclusions : These findings suggest that mechanical fluid flow activates guanylate cyclase. Mechanical fluid flow may enhance the proliferation of pulmonary epithelial cells and thereby facilitate the repair of the denuded epithelium in asthma. (Kitakanto Med J 2003 ; 53 : 257〜262)
- 北関東医学会の論文
- 2003-08-01
著者
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Mita Yoshinori
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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Mori M
Department Of Internal Medicine And Molecular Science Graduate School Of Medicine Gunma University
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SHIMIZU Yasuo
Department of Second Internal Medicine, Nagoya University School of Medicine
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Dobashi Kunio
Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
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Nakazawa Tsugio
Gunma University School of Health Sciences
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Moril Masatomo
Department of Medicine and Molecular Science (First Department of Internal Medicine), Gunma Universi
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Mori Masatomo
The First Department Of Internal Medicine Gunma University
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Makuuchi Masatoshi
The First Department Of Internal Medicine Gunma University School Of Medicine
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Miwa Yoshihiro
The Department Of Internal Medicine Numata National Hospital
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Dobashi Kunio
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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Shimizu Yasuo
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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Shimoyama Yasuyuki
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
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Shimizu Yasuo
Department Of Endoscopy And Endoscopic Surgery Gunma University Hospital
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Mori Masatomo
The First Department Of Internal Medicine
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Shimizu Yasuo
Department 4 Technology Research Division 2 Honda R&d Co. Ltd. Automobile R&d Center
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Dobashi Kunio
The First Department Of Internal Medicine
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Dobashi Kunio
The 1st Department Of Internal Medicine Gunma University School Of Medicine Faculty Of Medicine
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Nakazawa Tsugio
Gunma University Heavy Ion Medical Center
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Mita Yoshinori
Department of Internal Medicine, Public Tomioka General Hospital
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