Intercalibration of the Acridine Orange Direct Count Method of Aquatic Bacteria

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概要

• 論文の詳細を見る

• Intercalibration for counting planktonic bacteria by the acridine orange direct count (AODC) method was made among ten investigators of seven laboratories in Japan. The first calibration was made for one freshwater and two seawater samples sent to seven investigators belonging to five laboratories. Each investigator estimated bacterial abundance in these samples by the AODC method according to routine procedures of each laboratory. For the freshwater sample the estimates differed by a factor of 2.3 [coefficient of variation (CV), 36%], while for the seawater samples they varied greatly by factors of 4.0 and 11(CV's, 46 and 94%). In the second calibration nine investigators counted bacteria on the same AODC slides under the same optical condition. The estimates of nine investigators for three freshwater samples differed by factors of 1.8-2.5 (CV's, 24-29%), while for a seawater sample they differed by a factor of 17 (CV, 89%). Sampling error associated with contagious distribution of bacteria on a filter, and difference in the criterion for discriminating bacteria from nonbacterial particles were considered to be possible sources of variation in counting bacteria.

• 日本微生物生態学会の論文
• 1989-11-20

著者

• YAMAMOTO MAKOTO

  Department of Mathematics, Chuo University

• Fukui Manabu

  Department Of Biology Faculty Of Science Tokyo Metropolitan University

• Oh S‐w

  Shinshu Univ. Suwa Jpn

• Oh Su-wan

  Suwa Hydrobiological Station Shinshu University

• Nagata Toshi

  Otsu Hydrobiological Station Kyoto University

• Someya Takashi

  Division Of Microbiology School Of Medical Technology Universily Of Occupational And Environmental H

• Mori Masayuki

  Department Of Aging Angiology Research Cnter On Aging And Adaptation Shinshu University School Of Me

• Fukui Manabu

  Department Of Biological Science Graduate School Of Science Tokyo Metropolitan University

• KONDA TOSHIFUMI

  Department of Biology, Faculty of Science, Tokyo Metropolitan University

• KATO Kenji

  School of Allied Medical Sciences, Shinshu University

• MORIKAWA KAZUKO

  Laboratory of Biology, Tokyo University of Agriculture and Technology

• KURODA NOBUO

  Water Research Institute, Nagoya University

• TAKAHASHI KAZUSHI

  Water Research Institute, Nagoya University

• ARAKI SHIGEHIRO

  Department of Biology, Faculty of Science, Shinshu University

• Konda T

  National Inst. Infectious Disease Tokyo Jpn

• Konda Toshifumi

  Department Of Bacterial And Blood Products National Institute Of Infectious Diseases

• Mori Masayuki

  Department Of Biology Faculty Of Science Shinshu University

• Kuroda N

  Aichi Fisheries Res. Inst. Nagoya Jpn

• Kuroda Nobuo

  Water Research Institute Nagoya University

• Araki Shigehiro

  Department Of Biology Faculty Of Science Shinshu University

• Morikawa Kazuko

  Laboratory Of Biology Tokyo University Of Agriculture And Technology

• Takahashi Kazushi

  Water Research Institute Nagoya University

• Yamamoto Makoto

  Department Of Bacteriology National Institute Of Health

• Mori Masayuki

  Department Of Aging Angiology Research Center On Aging And Adaptation Shinshu University School Of M

• Kato Kenji

  School Of Allied Medical Sciences Shinshu University

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