中和剤を用いた改良最小殺菌濃度測定法による消毒薬耐性黄色ブドウ球菌の消毒薬感受性の評価

概要

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The susceptibilities of various antiseptics to Staphylcoccus aureus carrying antiseptic-resistance genes, qacA, smr and/or mutated nor A, were evaluated by a method to determine the minimum bactericidal concentration (MBC) and the minimum inhibitory concentration (MIC). In benzalkonium chloride, benzethonium chloride and alkydiaminoethylglycine hydrochloride, which are used for the washing of hands, skin-surfaces and wounds, we observed, in terms of MIC, no clear difference between the strains with and without antiseptic-resistance gene (s). However, we found that, in the modified method of MBC using a neutralizer, antiseptic-resistant strains survived after exposure to those antiseptics for 5 min at the minimum standard concentration (100μg/mL). The results showed that S. aureus strains carrying antiseptic-resistance gene (s) exhibited resistance to various antiseptics. Therefore, the modified determination of MBC was thus found to be useful for evaluating the susceptibility of antiseptics to antiseptic-resistant S. aureus.
日本医療薬学会の論文
2003-06-10