二枚貝の卵巣組繊による放卵数の推定

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In the present study, thirteen specimens of Cyclina sinensis were used for measuring volume of visceral mass and one of these specimens was used for counting the number of ripe eggs contained in ovarian tissue in spawning season. A piece of tissue was excised dorsoventrally through the visceral mass comprising the ovarian tissue, stomach and intestine. Tissue sections were cut at 8μm on a rotary microtome ; statistical analysis of ovarian tissue was performed on ten sections from the mid-region of the visceral mass. Sections through this region were representative of the distribution of reproductive tissues throughout the whole of the ovary. Sections were examined with compound microscope fitted with eyepiece graticule and then number of ripe eggs within the quadrate area of the micrometer was counted in the volume fraction of ten sections. From the ratio of ovarian tissue area (2.635 mm^2) within the quadrat of the micrometer to the area (30.5 mm^2) of tissue which is occupied by ovary in section, the number of ripe eggs contained in fraction of ten sections was estimated to be 575 ova. Assuming that the distribution of egg population throughout the whole of the visceral mass is similar to ovarian tissue within the quadrat of the eyepiece micrometer, the eggs that would be contained in an ovary may be about three million ova based on the mean volume (1.67629 cm^3) of thirteen visceral masses and about 2.4 million ova of 80% of ovarian ripe ova may be spawned.
福岡女子大学の論文
1990-01-25

二枚貝の卵巣組繊による放卵数の推定

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