Analysis of mariner-like elements in the smaller tea tortrix, Adoxophyes honmai and the summer fruit tortrix, Adoxophyes orana fasciata (Lepidoptera:Tortricidae)

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The mariner-like element(MLE) in two closely related leaf-roller moths, the smaller tea tortrix, Adoxophyes honmai and the summer fruit tortrix, Adoxophyes orana fasciata (Lepidoptera:Tortricidae) were surveyed. By a series of PCR and Southern blotting experiments, there were at least two types of MLEs in these two species. Sequence dissimilarity of each MLE suggests a possible polyphyletic origin and subsequent vertical inheritance within each species. In addition, a full-length MLE was isolated from a genomic library of A. honmai. This element, named Ahmarl, was 1, 294bp long with 27 bp inverted terminal repeats(ITRs). This element is apparently inactive due to a frameshift in the open reading frame(ORF) region, but a putative ORF could be obsevced by a single nucleotide removal, suggesting that the MLE in this species could have had the ability to transpose in the near past. Ahmarl may be suitable as a transformation vector with slight modification.
日本応用動物昆虫学会の論文
2000-08-25