Successful transformation of the housefly, Musca domestica(Diptera:Muscidae) with the transposable element, mariner
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概要
- 論文の詳細を見る
An active mariner transposable element(Mosl)was introduced into embryos of the housefly, Musca domestica. Twenty-six single G_0 males were crossed with normal virgin females. PCR assays using the Mosl specific primer were carried out in the G_1 generations, and DNA amplification was obtained in 4 lines (4/26). The Mosl element segregated in a Mendelian fashion from G_4 to G_5.In addition, PCR amplifications using two primer sets of the progeny in later generations indicated that the Mosl was integrated into the germlines of the housefly. These results demonstrate that mariner can serve as a germline transformation vector in the housefly and PCR detection is useful for species that lack a proper marker system.
- 日本応用動物昆虫学会の論文
- 2000-08-25
著者
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Honda H
Univ. Tsukuba Ibaraki Jpn
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Kimura K
Laboratory Of Apiculture Department Of Animal Genetics National Institute Of Animal Industry
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HONDA Hiroshi
Laboratory of Applied Entomology, Faculty of Agriculture, University of Tokyo
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Yoshiyama Mikio
Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba
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Kimura Kiyoshi
Laboratory of Apiculture, Department of Animal Genetics, National Institute of Animal Industry
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Kimura Kiyoshi
Laboratory Of Apiculture Department Of Animal Genetics National Institute Of Animal Industry
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Honda Hiroshi
Laboratory Of Applied Entomology Faculty Of Agriculture University Of Tokyo
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Yoshiyama Mikio
Laboratory Of Applied Zoology Institute Of Agriculture And Forestry University Of Tsukuba
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Honda Hiroshi
Laboratory Of Applied Entomology Faculty Of Agriculture The University Of Tokyo
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